| 质粒名称: | pENTR/pTER+ (430-1) |
|---|---|
| 出品公司: | 爱迪基因 |
| 目录编号: | 17453 |
| 存储实验室: | Eric Campeau, Paul Kaufman |
|
载体骨架基本信息 |
|
| 载体名称: | pENTR1A |
| 载体出品公司: | Invitrogen |
| 载体抗性: | 卡那霉素 |
| 空载体大小: | 3313 bp |
| 载体类型: | RNAi ; Entry vector |
| 高拷贝/低拷贝: | 高拷贝 |
| 生长条件: | 37 ℃ |
|
插入基因信息 |
|
| 插入基因名称: | None |
| 克隆方法: | 限制性内切酶 |
| 5' 测序引物 | ENTRforw |
| 3' 测序引物 | ENTRrev |
|
文献引用方法 |
|
| 材料和方法部分: | pENTR/pTER+ (430-1) was a gift from Eric Campeau & Paul Kaufman (爱迪基因 plasmid # 17453) |
| 参考文献部分: | A versatile viral system for expression and depletion of proteins in mammalian cells. Campeau E, Ruhl VE, Rodier F, Smith CL, Rahmberg BL, Fuss JO, Campisi J, Yaswen P, Cooper PK, Kaufman PD.. PLoS One. 2009 Aug 6;4(8):e6529. 10.1371/journal.pone.0006529 PubMed 19657394 |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
质粒图谱.png)
载体简介
载体序列
LOCUS pENTR/pTER+ (430-1) 3313 bp ds-DNA circular SYN 10-JAN-2018
DEFINITION Gateway entry vector for shRNA expression under H1/TO promoter.
ACCESSION .
VERSION .
KEYWORDS pENTR/pTER+ (430-1)
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 3313)
AUTHORS Campeau E, Ruhl VE, Rodier F, Smith CL, Rahmberg BL, Fuss JO,
Campisi J, Yaswen P, Cooper PK, Kaufman PD.
TITLE A versatile viral system for expression and depletion of proteins in
mammalian cells.
JOURNAL PLoS One. 2009 Aug 6;4(8):e6529.
PUBMED 19657394
COMMENT This file is created by Vector NTI
http://www.biofeng.com/
COMMENT ORIGDB|GenBank
COMMENT VNTAUTHORNAME|biofeng.com|
COMMENT VNTNAME|pENTR/pTER+ (430-1)|
FEATURES Location/Qualifiers
source 1..3313
/organism="synthetic DNA construct"
/mol_type="other DNA"
terminator 103..189
/gene="Escherichia coli rrnB"
/label=rrnB T1 terminator
/note="transcription terminator T1 from the E. coli rrnB
gene"
terminator 281..308
/label=rrnB T2 terminator
/note="transcription terminator T2 from the E. coli rrnB
gene"
primer_bind 316..339
/label=pENTR-F
/note="pENTR vectors, forward primer"
protein_bind 358..457
/gene="mutant version of attL"
/label=attL1
/bound_moiety="LR Clonase(TM)"
/note="recombination site for the Gateway(R) LR reaction"
primer_bind 634..653
/label=H1
/note="Human H1 promoter, forward primer"
protein_bind 699..717
/gene="tetO"
/label=tet operator
/bound_moiety="tetracycline repressor TetR"
/note="bacterial operator O2 for the tetR and tetA genes"
protein_bind complement(1542..1641)
/gene="mutant version of attL"
/label=attL2
/bound_moiety="LR Clonase(TM)"
/note="recombination site for the Gateway(R) LR reaction"
primer_bind complement(1753..1772)
/label=pENTR-R
/note="pENTR vectors, reverse primer"
CDS 1764..2573
/codon_start=1
/gene="aph(3')-Ia"
/product="aminoglycoside phosphotransferase"
/label=KanR
/note="confers resistance to kanamycin in bacteria or G418
(Geneticin(R)) in eukaryotes"
/translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
FQVLEEYPDSGENIVDALAVSLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
primer_bind complement(1831..1850)
/label=Kan-R
/note="Kanamycin resistance gene, reverse primer"
rep_origin 2663..3251
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind 3152..3171
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
ORIGIN
1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgctagca tggatctcgg
61 ggacgtctaa ctactaagcg agagtaggga actgccaggc atcaaataaa acgaaaggct
121 cagtcggaag actgggcctt tcgttttatc tgttgtttgt cggtgaacgc tctcctgagt
181 aggacaaatc cgccgggagc ggatttgaac gttgtgaagc aacggcccgg agggtggcgg
241 gcaggacgcc cgccataaac tgccaggcat caaactaagc agaaggccat cctgacggat
301 ggcctttttg cgtttctaca aactcttcct gttagttagt tacttaagct cgggccccaa
361 ataatgattt tattttgact gatagtgacc tgttcgttgc aacaaattga taagcaatgc
421 ttttttataa tgccaacttt gtacaaaaaa gcaggcttta aaggaaccaa ttcagtcgac
481 tggatccggt accgaattcg aacgctgacg tcatcaaccc gctccaagga atcgcggccc
541 agtgtcacta ggcgggaaca cccagcgcgc gtgcgccctg gcaggaagat ggctgtgagg
601 gacaggggag tggcgccctg caatatttgc atgtcgctat gtgttctggg aaatcaccat
661 aaacgtgaaa tgtctttgga tttgggaatc ttataagttc cctatcagtg atagagatct
721 cggcgttgtg catgtcgggc gactgctcca tgatcttgcg ccgctcgatc tgcgaccaca
781 ccataaaggc gttcatgggc cgcttgatgt ggccactggg cgtcttgcac cagctggggt
841 cgtccgcctt gccgcccgtc gacgcggtgg agccaggcgt cggggaggac gcgatgccca
901 gctccaggcc ggcgcccgag tccgagctct ccccggccag cagagcctca gtgttctccg
961 cgttgttggt ctgttgtacc atggccccgg cttgccgggc gcccacgcgc gctcacacgc
1021 tcgcggcgcg gtcgccgggc cctgcgggct ccccggggcg gagaccaggg tcccctgagc
1081 ggcgcgcggg ccaggggcgg ccgcctcccg ccgccaacct agagtccccg aggccctccg
1141 tcctccctca ccgctgaagt gctccctgcg ggcaccgagg ctggagtcgg ccgcggcgag
1201 gagtttctcc aaacgttggt agccggagta tcttccaaac gcaagtttct cgccgcctcc
1261 ggggcaagtc tttcccccac ccctttccct gaagcagtcg attgcagttc acgagagctc
1321 gcggtagctc aggaaagcga catcgtctct agcgcctaag tccctttcct gcagtgcaaa
1381 gccaaagcga ctctggctcc gggactctct gtgggcggaa tcggcgctga ggagttggtg
1441 caattatttt gttgcaaggt aggaagccaa gaagcttggt accgagctcg gatccactag
1501 tccagtgtgg tggaattcgc ggccgcactc gagatatcta gacccagctt tcttgtacaa
1561 agttggcatt ataagaaagc attgcttatc aatttgttgc aacgaacagg tcactatcag
1621 tcaaaataaa atcattattt gccatccagc tgcagctctg gcccgtgtct caaaatctct
1681 gatgttacat tgcacaagat aaaaatatat catcatgaac aataaaactg tctgcttaca
1741 taaacagtaa tacaaggggt gttatgagcc atattcaacg ggaaacgtcg aggccgcgat
1801 taaattccaa catggatgct gatttatatg ggtataaatg ggctcgcgat aatgtcgggc
1861 aatcaggtgc gacaatctat cgcttgtatg ggaagcccga tgcgccagag ttgtttctga
1921 aacatggcaa aggtagcgtt gccaatgatg ttacagatga gatggtcaga ctaaactggc
1981 tgacggaatt tatgcctctt ccgaccatca agcattttat ccgtactcct gatgatgcat
2041 ggttactcac cactgcgatc cccggaaaaa cagcattcca ggtattagaa gaatatcctg
2101 attcaggtga aaatattgtt gatgcgctgg cagtgtccct gcgccggttg cattcgattc
2161 ctgtttgtaa ttgtcctttt aacagcgatc gcgtatttcg tctcgctcag gcgcaatcac
2221 gaatgaataa cggtttggtt gatgcgagtg attttgatga cgagcgtaat ggctggcctg
2281 ttgaacaagt ctggaaagaa atgcataaac ttttgccatt ctcaccggat tcagtcgtca
2341 ctcatggtga tttctcactt gataacctta tttttgacga ggggaaatta ataggttgta
2401 ttgatgttgg acgagtcgga atcgcagacc gataccagga tcttgccatc ctatggaact
2461 gcctcggtga gttttctcct tcattacaga aacggctttt tcaaaaatat ggtattgata
2521 atcctgatat gaataaattg cagtttcatt tgatgctcga tgagtttttc taatcagaat
2581 tggttaattg gttgtaacat tattcagatt gggccccgtt ccactgagcg tcagacccgg
2641 tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc
2701 aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc
2761 tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtt cttctagtgt
2821 agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc
2881 taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact
2941 caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac
3001 agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag
3061 aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg
3121 gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg
3181 tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga
3241 gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt
3301 ttgctcacat gtt
//
